Polysaccharide Biosynthesis: Glycosyltransferases and Their Complexes

Glycosyltransferases (GTs) are enzymes that catalyze reactions attaching an activated sugar to an acceptor substrate, which may be a polysaccharide, peptide, lipid, or small molecule. In the past decade, notable progress has been made in revealing and cloning genes encoding polysaccharide-synthesizing GTs. However, the vast majority of GTs remain structurally and functionally uncharacterized. The mechanism by which they are organized in the Golgi membrane, where they synthesize complex, highly branched polysaccharide structures with high efficiency and fidelity, is also mostly unknown. This review will focus on current knowledge about plant polysaccharide-synthesizing GTs, specifically focusing on protein-protein interactions and the formation of multiprotein complexes.

Automated summary

Glycosyltransferases (GTs) are enzymes that attach activated sugar to acceptor substrates. While progress has been made in cloning genes encoding polysaccharide-synthesizing GTs, the majority of GTs remain uncharacterized. The mechanism by which they synthesize complex polysaccharide structures in the Golgi membrane is largely unknown.