Dissolved organic matter as a terminal electron acceptor in the microbial oxidation of steroid estrogen

Steroid estrogen in natural waters may be biodegraded by quinone-reducing bacteria, dissolved organic matter (DOM) may serve as a terminal electron acceptor in this process. The influence of temperature, pH, dissolved oxygen and light illumination on the reduction efficiency of anthraquinone-2-disulfonate (AQS) was investigated using 17 beta-estradiol (E2) as the target species. The optimum reduction conditions were found to be in the dark under anaerobic conditions at pH 8.0 and 30 degrees C. Quinone-reducing bacteria can use the quinone structure of DOM components as a terminal electron acceptor coupling with microbial growth to promote biodegradation. Compared with other DOM models, AQS best stimulated E2 biodegradation and the mediating effect was improved as the AQS concentration increased from 0 to 0.5 mM. However, further increase had an inhibiting effect. Natural DOM containing lake humic acid (LHA) and lake fulvic acid (LFA) had a very important accelerating effect on the degradation of E2, the action mechanism of which was consistent with that defined using DOM models. The natural DOM contained more aromatic compounds, demonstrating their greater electron-accepting capacity and generally more effective support for microorganism growth and E2 oxidation than Aldrich humic acid (HA). These results provide a more comprehensive understanding of microbial degradation of steroid estrogens in anaerobic environments and confirm DOM as an important terminal electron acceptor in pollutant transformation. (C) 2016 Elsevier Ltd. All rights reserved.