Journal
FRONTIERS IN MICROBIOLOGY
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2021.619731
Keywords
chitosanase; chitooligosaccharides; deep-sea bacterium; Serratia sp; QD07; fermenter
Categories
Funding
- National Natural Science Foundation of China [81473384]
- Natural Science Foundation of Shandong Province [ZR2018BH036]
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Chitosanase CsnS, cloned from a deep-sea bacterium and over-expressed in E. coli, is a cold-adapted enzyme with high activity at 60°C. It exhibits an endo-type cleavage pattern and can hydrolyze chitosan polymers to yield disaccharides and trisaccharides, making it a potential candidate for industrial production of COS.
Chitosanase is a significant chitosan-degrading enzyme involved in industrial applications, which forms chitooligosaccharides (COS) as reaction products that are known to have various biological activities. In this study, the gene csnS was cloned from a deep-sea bacterium Serratia sp. QD07, as well as over-expressed in Escherichia coli, which is a new chitosanase encoding gene. The recombinant strain was cultured in a 5 L fermenter, which yielded 324 U/mL chitosanases. After purification, CsnS is a cold-adapted enzyme with the highest activity at 60 degrees C, showing 37.5% of the maximal activity at 0 degrees C and 42.6% of the maximal activity at 10 degrees C. It exhibited optimum activity at pH 5.8 and was stable at a pH range of 3.4-8.8. Additionally, CsnS exhibited an endo-type cleavage pattern and hydrolyzed chitosan polymers to yield disaccharides and trisaccharides as the primary reaction products. These results make CsnS a potential candidate for the industrial manufacture of COS.
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