4.7 Article

Development of an Immunofluorescence Assay Module for Determination of the Mycotoxin Zearalenone in Water

Journal

TOXINS
Volume 13, Issue 3, Pages -

Publisher

MDPI
DOI: 10.3390/toxins13030182

Keywords

zearalenone; mycotoxin; competitive immunoassay; fluorescence detection; high-performance liquid chromatography; total internal reflection ellipsometry

Funding

  1. Hungarian National Research, Development and Innovation Office within the National Competitiveness and Excellence Program [NVKP_16-1-2016-0049]

Ask authors/readers for more resources

Project Aquafluosense is focused on developing prototypes for a fluorescence-based instrumentation setup for in situ measurements of various water quality parameters, with a specific emphasis on detecting emerging pollutants like ZON. The ELFIA method developed in this project shows high sensitivity in detecting environmental xenobiotics, with the 96-well microplate-based fluorescence instrument being capable of detecting ZON in a concentration range of 0.09-400 ng/mL. This method demonstrates efficacy in combined in situ applications for determining important water quality parameters through induced fluorimetry.
Project Aquafluosense is designed to develop prototypes for a fluorescence-based instrumentation setup for in situ measurements of several characteristic parameters of water quality. In the scope of the project an enzyme-linked fluorescent immunoassay (ELFIA) method has been developed for the detection of several environmental xenobiotics, including mycotoxin zearalenone (ZON). ZON, produced by several plant pathogenic Fusarium species, has recently been identified as an emerging pollutant in surface water, presenting a hazard to aquatic ecosystems. Due to its physico-chemical properties, detection of ZON at low concentrations in surface water is a challenging task. The 96-well microplate-based fluorescence instrument is capable of detecting ZON in the concentration range of 0.09-400 ng/mL. The sensitivity and accuracy of the analytical method has been demonstrated by a comparative assessment with detection by high-performance liquid chromatography and by total internal reflection ellipsometry. The limit of detection of the method, 0.09 ng/mL, falls in the low range compared to the other reported immunoassays, but the main advantage of this ELFIA method is its efficacy in combined in situ applications for determination of various important water quality parameters detectable by induced fluorimerty-e.g., total organic carbon content, algal density or the level of other organic micropollutants detectable by immunofluorimetry. In addition, the immunofluorescence module can readily be expanded to other target analytes if proper antibodies are available for detection.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available