Journal
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
Volume 18, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/ijerph18052412
Keywords
intercalation; external binding; transport; inner filter effect; micelles; liposomes; hydrophobicity; enthalpy-entropy compensation
Funding
- National Research in Antarctica [PNRA16_00020-A1, PNRA18_00194, PNRA18_00216]
- la Caixa Foundation [LCF/PR/PR12/11070003]
- Ministerio de Ciencia, Innovacion y Universidades-FEDER [RTI2018-102040-B-100]
- Junta de Castilla y Leon-FEDER [BU305P18]
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The study investigated the interaction of paraquat and diquat herbicides with DNA and protein using multiple experimental methods. The herbicides showed low affinity for DNA oligonucleotides but strong binding to bovine serum albumin. The results suggest that the herbicides may have optimal conditions for transport in the body.
The study of the interaction of persistent organic pollutants with biosubstrates helps to unravel the pathways for toxicity, however, few mechanistic data are present in the literature for these systems. We analyzed the binding of paraquat (PQ) and diquat (DQ) herbicides to natural calf thymus DNA and a DNA G-quadruplex by spectrophotometric titrations, ethidium bromide exchange tests, viscometry, and melting experiments. The interaction with bovine serum albumin (BSA) protein was studied spectrofluorimetrically at different temperatures. The retention of the targets on positive, negative, and neutral micellar aggregates and liposomes was analyzed by ultrafiltration experiments. Despite some favorable features, PQ and DQ only externally bind natural DNA and do not interact with DNA oligonucleotides. Both herbicides bind bovine serum albumin (BSA). PQ binds BSA mainly according to an electrostatics-driven process. However, ultrafiltration data also show that some hydrophobic contribution participates in the features of these systems. The practical problems related to unfavorable spectroscopic signals and inner filter effects are also discussed. Overall, both herbicides show a low affinity for nucleic acids and weak penetration into liposomes; in addition, the equilibrium constants values found for BSA system suggest optimal conditions for transport in the body.
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