Journal
CELL REPORTS
Volume 34, Issue 5, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2021.108722
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Funding
- DFG [Vo875-19/1, GRK 2157/1]
- Interdisciplinary Center for Clinical Research Wurzburg [IZKF Z-6]
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The study reveals the important role of the Hfq-associated sRNA PinT in Salmonella virulence, showing its translational inhibition of the secreted effector kinase SteC and delay of actin rearrangements in infected host cells. The findings highlight the need for complementary methods to uncover the full target suites of sRNAs.
A full understanding of the contribution of small RNAs (sRNAs) to bacterial virulence demands knowledge of their target suites under infection-relevant conditions. Here, we take an integrative approach to capturing targets of the Hfq-associated sRNA PinT, a known post-transcriptional timer of the two major virulence programs of Salmonella enterica. Using MS2 affinity purification and RNA sequencing (MAPS), we identify PinT ligands in bacteria under in vitro conditions mimicking specific stages of the infection cycle and in bacteria growing inside macrophages. This reveals PinT-mediated translational inhibition of the secreted effector kinase SteC, which had gone unnoticed in previous target searches. Using genetic, biochemical, and microscopic assays, we provide evidence for PinT-mediated repression of steC mRNA, eventually delaying actin rearrangements in infected host cells. Our findings support the role of PinT as a central post-transcriptional regulator in Salmonella virulence and illustrate the need for complementary methods to reveal the full target suites of sRNAs.
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