Journal
THORACIC CANCER
Volume 12, Issue 10, Pages 1495-1502Publisher
WILEY
DOI: 10.1111/1759-7714.13928
Keywords
FAM83A; FAM83A‐ AS1; lncRNA; LUAD
Categories
Funding
- National Natural Science Foundation of China [81672934, 81873417]
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In this study, it was found that high expression of FAM83A-AS1 in non-small cell lung cancer was closely associated with low overall survival and progression-free survival. Functionally, high FAM83A-AS1 expression increased lung adenocarcinoma cell proliferation and metastasis, indicating its oncogenic functions. Mechanistically, FAM83A-AS1 enhanced FAM83A expression by increasing pre-mRNA stability and promoted tumorigenesis of lung adenocarcinoma.
Background Lung cancer is the leading cause of cancer deaths worldwide. Long non-coding RNAs (lncRNAs) affect a series of cellular biological processes, including oncogene function promotion. In this study, we explored the functions and mechanisms of FAM83A antisense RNA 1 (FAM83A-AS1) in non-small cell lung cancer (NSCLC) progression. Methods The expression of FAM83A-AS1and FAM83A mRNA was analyzed using the Cancer Genome Atlas (TCGA) data. Proliferation, migration, invasion and Western blotting were measured after treatment with overexpressed or knockdown FAM83A-AS1. To determine the relationship between FAM83A-AS1 and FAM83A, RNase protection assay (RPA), amanitin treatment, RNA pulldown assay and RNA immunoprecipitation (RIP) assay were performed. Results High expression of FAM83A-AS1 in lung adenocarcinoma (LUAD) was closely associated with low overall survival (OS) and progression-free survival (PFS). Functionally, high FAM83A-AS1 expression increased LUAD cell proliferation and metastasis, indicating that FAM83A-AS1 exerted its oncogenic functions. Furthermore, FAM83A-AS1 promoted NSCLC progression via ERK signaling pathways. Mechanistically, FAM83A-AS1 post-transcriptionally increased FAM83A expression by enhancing pre-mRNA stability. FAM83A-AS1 enhanced FAM83A mRNA stability not only by forming an RNA duplex but also by binding to FBL. Conclusions We determined that FAM83A-AS1 increased FAM83A expression by enhancing FAM83A pre-mRNA stability and promoted the tumorigenesis of LUAD.
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