Journal
STEM CELL RESEARCH & THERAPY
Volume 12, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s13287-021-02184-1
Keywords
Inner ear; Spiral ganglion neurons; Neurogenin1; Blastocyst complementation; Stem cells; Regenerative medicine
Funding
- National Institute on Deafness and Other Communication Disorders of the National Institutes of Health [F31DC015153]
- National Institute on Aging of the National Institute of Health [2T32AG029796-11]
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This study successfully produced induced pluripotent stem cell-derived inner ear sensory neurons in a mouse model using blastocyst complementation. The approach not only corrected non-sensory deficits associated with Neurog1 heterozygosity, but also highlighted blastocyst complementation as a potential tool for creating chimeric inner ear cell types for transplantation into damaged inner ears to improve hearing.
This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1(+/-) heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing.
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