Journal
SCIENTIFIC REPORTS
Volume 11, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41598-021-83462-z
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Funding
- Swedish Foundation for Strategic Environmental Research MISTRA
- Swedish Governmental Research program Trees and Crops for the Future (TC4F)
- Formas
- Swedish government research council for sustainable development [2018-01459]
- Lyckeby Research Foundation (Stiftelsen Starkelsen Forskning Utveckling)
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DNA-free genome editing was used to induce mutations in branching enzyme genes of tetraploid potato, leading to the development of starch varieties with altered properties. The mutations resulted in significant changes in crystalline structure, chain length distribution, and granule phenotype of the starch.
DNA-free genome editing was used to induce mutations in one or two branching enzyme genes (Sbe) in tetraploid potato to develop starch with an increased amylose ratio and elongated amylopectin chains. By using ribonucleoprotein (RNP) transfection of potato protoplasts, a mutation frequency up to 72% was achieved. The large variation of mutations was grouped as follows: Group 1 lines with all alleles of Sbe1 mutated, Group 2 lines with all alleles of Sbe1 as well as two to three alleles of Sbe2 mutated and Group 3 lines having all alleles of both genes mutated. Starch from lines in Group 3 was found to be essentially free of amylopectin with no detectable branching and a chain length (CL) distribution where not only the major amylopectin fraction but also the shortest amylose chains were lost. Surprisingly, the starch still formed granules in a low-ordered crystalline structure. Starch from lines of Group 2 had an increased CL with a higher proportion of intermediate-sized chains, an altered granule phenotype but a crystalline structure in the granules similar to wild-type starch. Minor changes in CL could also be detected for the Group 1 starches when studied at a higher resolution.
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