Effects of a single aerobic exercise on perfused boundary region and microvascular perfusion: a field study

The endothelium and the glycocalyx play a pivotal role in regulating microvascular function and perfusion in health and critical illness. It is unknown today, whether aerobic exercise immediately affects dimensions of the endothelial surface layer (ESL) in relation to microvascular perfusion as a physiologic adaption to increased nutritional demands. This monocentric observational study was designed to determine real-time ESL and perfusion measurements of the sublingual microcirculation using sidestream dark field imaging performed in 14 healthy subjects before and after completing a 10 km trial running distance. A novel image acquisition and analysis software automatically analysed the perfused boundary region (PBR), an inverse parameter for red blood cell (RBC) penetration of the ESL, in vessels between 5 and 25 mu m diameter. Microvascular perfusion was assessed by calculating RBC filling percentage. There was no significant immediate effect of exercise on PBR and RBC filling percentage. Linear regression analysis revealed a distinct association between change of PBR and change of RBC filling percentage (regression coefficient beta: - 0.026; 95% confidence interval - 0.043 to - 0.009; p = 0.006). A single aerobic exercise did not induce a change of PBR or RBC filling percentage. The endothelium of the microvasculature facilitates efficient perfusion in vessels reacting with an increased endothelial surface layer.

Automated summary

This study examined the real-time ESL and perfusion measurements of the sublingual microcirculation in healthy subjects before and after a 10 km running distance. The results showed no immediate effect of exercise on PBR and RBC filling percentage, but a significant association between changes in PBR and RBC filling percentage was observed. Aerobic exercise did not induce changes in PBR or RBC filling percentage, suggesting that the endothelium of microvasculature facilitates efficient perfusion in vessels reacting with an increased endothelial surface layer.