Journal
NATURE COMMUNICATIONS
Volume 12, Issue 1, Pages -Publisher
NATURE RESEARCH
DOI: 10.1038/s41467-021-21695-2
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Funding
- Medical Research Council [MC_U105184308, MC_U10517878, MC_UP_1201/16]
- Cancer Research UK [C14801/A21211]
- European Research Council (ERC) under the European Union [ERC-CoG-648432]
- German Research Foundation DFG [SFB1035, Sonderforschungsbereich 1035, 201302640]
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) [392923329]
- Wellcome Trust [103139, 203149]
- MRC [MC_U105184308] Funding Source: UKRI
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The lipid phosphatidylinositol-3-phosphate (PI3P) acts as a regulator of endocytosis and autophagy, generated by two complexes containing the lipid kinase VPS34. Rab5 and Rab1a GTPases recruit and activate specific VPS34 complexes on membranes through unique binding interfaces, guiding them to distinct cellular locations. Insights into the recruitment and activation mechanisms of VPS34 complexes by specific Rab GTPases are provided through biochemical and structural analyses.
The lipid phosphatidylinositol-3-phosphate (PI3P) is a regulator of two fundamental but distinct cellular processes, endocytosis and autophagy, so its generation needs to be under precise temporal and spatial control. PI3P is generated by two complexes that both contain the lipid kinase VPS34: complex II on endosomes (VPS34/VPS15/Beclin 1/UVRAG), and complex I on autophagosomes (VPS34/VPS15/Beclin 1/ATG14L). The endosomal GTPase Rab5 binds complex II, but the mechanism of VPS34 activation by Rab5 has remained elusive, and no GTPase is known to bind complex I. Here we show that Rab5a-GTP recruits endocytic complex II to membranes and activates it by binding between the VPS34 C2 and VPS15 WD40 domains. Electron cryotomography of complex II on Rab5a-decorated vesicles shows that the VPS34 kinase domain is released from inhibition by VPS15 and hovers over the lipid bilayer, poised for catalysis. We also show that the GTPase Rab1a, which is known to be involved in autophagy, recruits and activates the autophagy-specific complex I, but not complex II. Both Rabs bind to the same VPS34 interface but in a manner unique for each. These findings reveal how VPS34 complexes are activated on membranes by specific Rab GTPases and how they are recruited to unique cellular locations. The phosphatidylinositol-3-phosphate (PI3P) is generated by the lipid kinase VPS34, in the context of VPS34 complex I on autophagosomes or complex II on endosomes. Biochemical and structural analyses provide insights into the mechanism of both VPS34 complexes recruitment to and activation on membranes by specific Rab GTPases.
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