Journal
NATURE COMMUNICATIONS
Volume 12, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41467-021-21559-9
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Funding
- Agency for Science, Technology and Research (A*STAR)
- Advanced Manufacturing and Engineering (AME) Programmatic grant [A18A9b0060]
- Industrial Alignment Fund Pre-Positioning (IAF-PP) grant [H17/01/a0/012]
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A new class of base editors, C:G to G:C Base Editors, have been developed in this study to create single-base genomic transversions in human cells, targeting specific genes related to genetic diseases. These editors predominantly perform C:G to G:C editing with high purity and efficiency, showing therapeutic potential for interrogating and correcting human genetic diseases.
Many genetic diseases are caused by single-nucleotide polymorphisms. Base editors can correct these mutations at single-nucleotide resolution, but until recently, only allowed for transition edits, addressing four out of twelve possible DNA base substitutions. Here, we develop a class of C:G to G:C Base Editors to create single-base genomic transversions in human cells. Our C:G to G:C Base Editors consist of a nickase-Cas9 fused to a cytidine deaminase and base excision repair proteins. Characterization of >30 base editor candidates reveal that they predominantly perform C:G to G:C editing (up to 90% purity), with rAPOBEC-nCas9-rXRCC1 being the most efficient (mean 15.4% and up to 37% without selection). C:G to G:C Base Editors target cytidine in WCW, ACC or GCT sequence contexts and within a precise three-nucleotide window of the target protospacer. We further target genes linked to dyslipidemia, hypertrophic cardiomyopathy, and deafness, showing the therapeutic potential of these base editors in interrogating and correcting human genetic diseases. Many diseases are caused by single-nucleotide polymorphisms. Here, the authors present CRISPR base editors that use the base excision machinery for single-base transversions.
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