4.8 Article

Proteome-wide and matrisome-specific alterations during human pancreas development and maturation

Journal

NATURE COMMUNICATIONS
Volume 12, Issue 1, Pages -

Publisher

NATURE RESEARCH
DOI: 10.1038/s41467-021-21261-w

Keywords

-

Funding

  1. NIH [R21AI126419, R01DK071801, RF1AG052324, P41GM108538, 1F31DK125021-01, NIH-NCRR S10RR029531]
  2. Juvenile Diabetes Research Foundation [1-PNF-2016-250-S-B, SRA-2016-168-S-B]
  3. NIH/NCATS through the University of Wisconsin Institute for Clinical and Translational Research [UL1TR002373]
  4. Office of the Vice Chancellor for Research and Graduate Education at the University of WisconsinMadison
  5. Wisconsin Alumni Research Foundation
  6. University of Wisconsin-Madison School of Pharmacy

Ask authors/readers for more resources

This study used mass spectrometry to analyze the ECM proteome of the human pancreas at different age stages, identifying new matrisome features and visualizing specific ECM proteins of interest through immunofluorescent staining. The research contributes to a better understanding of the critical roles that ECM plays throughout human pancreas development and maturation.
The extracellular matrix (ECM) is unique to each tissue and capable of guiding cell differentiation, migration, morphology, and function. The ECM proteome of different developmental stages has not been systematically studied in the human pancreas. In this study, we apply mass spectrometry-based quantitative proteomics strategies using N,N-dimethyl leucine isobaric tags to delineate proteome-wide and ECM-specific alterations in four age groups: fetal (18-20 weeks gestation), juvenile (5-16 years old), young adults (21-29 years old) and older adults (50-61 years old). We identify 3,523 proteins including 185 ECM proteins and quantify 117 of them. We detect previously unknown proteome and matrisome features during pancreas development and maturation. We also visualize specific ECM proteins of interest using immunofluorescent staining and investigate changes in ECM localization within islet or acinar compartments. This comprehensive proteomics analysis contributes to an improved understanding of the critical roles that ECM plays throughout human pancreas development and maturation.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available