Journal
EPIGENOMICS
Volume 13, Issue 6, Pages 411-422Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/epi-2020-0345
Keywords
human cardiomyocytes; lipopolysaccharide; long noncoding RNA; myocarditis; NONHSAT177112; 1
Categories
Funding
- National Natural Science Foundation of China [8187020860]
- Science and Technology Development Plan of Shandong Province [2017WS427]
- Tai'an Science and Technology Development Plan [2017NS0204]
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In this study, the researchers investigated the role of lncRNA NONHSAT177112.1 in inflammatory injury of human cardiomyocytes induced by lipopolysaccharide. Results showed that NONHSAT177112.1 is expressed in the nucleus and cytoplasm, with dynamic expression patterns during LPS stimulation. Knocking down NONHSAT177112.1 reversed the promotion effect of LPS on inflammatory injury, while overexpression had the opposite effects.
Aim: To explore the roles of lncRNA NONHSAT177112.1 in the inflammatory injury of human cardiomyocytes (HCMs) induced by lipopolysaccharide (LPS). Materials & methods: The sublocalization of NONHSAT177112.1 was detected by FISH. HCMs were stimulated with LPS to induce inflammatory injury. NONHSAT177112.1 expression was detected by quantitative real-time PCR. Cell apoptosis and viability were detected by flow cytometry and CCK-8 assays. The expression of inflammatory cytokines and myocardial enzymes were detected by PCR and ELISA. Results: NONHSAT177112.1 is expressed in the nucleus and cytoplasm. NONHSAT177112.1 showed dynamic expression that first increased and then decreased during LPS stimulation. NONHSAT177112.1 knockdown reversed the promotion effect of LPS on inflammatory injury. Conversely, NONHSAT177112.1 overexpression exerted the opposite effects. Conclusion: NONHSAT177112.1 aggravates inflammatory injury in LPS-treated HCMs.
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