Journal
VIRUSES-BASEL
Volume 13, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/v13030401
Keywords
influenza C virus; escape mutant; antigenic drift; growth kinetics; surveillance
Categories
Funding
- Japan Society for the Promotion of Science (JSPS) KAKENHI [18K07783]
- Grants-in-Aid for Scientific Research [18K07783] Funding Source: KAKEN
Ask authors/readers for more resources
The study on the antigenicity of the hemagglutinin esterase glycoprotein of influenza C virus revealed that mutations in different antigenic sites can affect viral replication, and certain residues are likely to mediate antigenic drift while maintaining replicative ability.
The antigenicity of the hemagglutinin esterase (HE) glycoprotein of influenza C virus is known to be stable; however, information about residues related to antigenic changes has not yet been fully acquired. Using selection with anti-HE monoclonal antibodies, we previously obtained some escape mutants and identified four antigenic sites, namely, A-1, A-2, A-3, and Y-1. To confirm whether the residues identified as the neutralizing epitope possibly relate to the antigenic drift, we analyzed the growth kinetics of these mutants. The results showed that some viruses with mutations in antigenic site A-1 were able to replicate to titers comparable to that of the wild-type, while others showed reduced titers. The mutants possessing substitutions in the A-2 or A-3 site replicated as efficiently as the wild-type virus. Although the mutant containing a deletion at positions 192 to 195 in the Y-1 site showed lower titers than the wild-type virus, it was confirmed that this region in the 190-loop on the top side of the HE protein is not essential for viral propagation. Then, we revealed that antigenic changes due to substitutions in the A-1, A-3, and/or Y-1 site had occurred in nature in Japan for the past 30 years. These results suggest that some residues (i.e., 125, 176, 192) in the A-1 site, residue 198 in the A-3 site, and residue 190 in the Y-1 site are likely to mediate antigenic drift while maintaining replicative ability.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available