Journal
XENOTRANSPLANTATION
Volume 28, Issue 3, Pages -Publisher
WILEY
DOI: 10.1111/xen.12666
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Funding
- OPOStiftung
- Child foundation
- foundation La Colombe in Geneva
- CTI Swiss National Science Foundation [310030E-164520]
- Foundation Insuleman
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Neonatal and juvenile porcine islet cell clusters (ICC) are considered as an unlimited source for islet xenotransplantation to treat type 1 diabetes patients. Multipotent mesenchymal stromal cells derived from the same pancreata (pMSC) were found to improve insulin secretion of ICC through direct cell-cell contact, indicating a potential role in supporting beta-cell function in transplantation. Further development of new anti-fibrotic polymers or genetically modified pigs with lower immunogenicity may be necessary for successful clinical application.
Neonatal and juvenile porcine islet cell clusters (ICC) present an unlimited source for islet xenotransplantation to treat type 1 diabetes patients. We isolated ICC from pancreata of 14 days old juvenile piglets and characterized their maturation by immunofluorescence and insulin secretion assays. Multipotent mesenchymal stromal cells derived from exocrine tissue of same pancreata (pMSC) were characterized for their differentiation potential and ability to sustain ICC insulin secretion in vitro and in vivo. Isolation of ICC resulted in 142 +/- 50 x 10(3) IEQ per pancreas. Immunofluorescence staining revealed increasing presence of insulin-positive beta cells between day 9 and 21 in culture and insulin content per 500IEC of ICC increased progressively over time from 1178.4 +/- 450 mu g/L to 4479.7 +/- 1954.2 mu g/L from day 7 to 14, P < .001. Highest glucose-induced insulin secretion by ICC was obtained at day 7 of culture and reached a fold increase of 2.9 +/- 0.4 compared to basal. Expansion of adherent cells from the pig exocrine tissue resulted in a homogenous CD90(+), CD34(-), and CD45(-) fibroblast-like cell population and differentiation into adipocytes and chondrocytes demonstrated their multipotency. Insulin release from ICC was increased in the presence of pMSC and dependent on cell-cell contact (glucose-induced fold increase: ICC alone: 1.6 +/- 0.2; ICC + pMSC + contact: 3.2 +/- 0.5, P = .0057; ICC + pMSC no-contact: 1.9 +/- 0.3; theophylline stimulation: alone: 5.4 +/- 0.7; pMSC + contact: 8.4 +/- 0.9, P = .013; pMSC no-contact: 5.2 +/- 0.7). After transplantation of encapsulated ICC using Ca2+-alginate (alg) microcapsules into streptozotocin-induced diabetic and immunocompetent mice, transient normalization of glycemia was obtained up to day 7 post-transplant, whereas ICC co-encapsulated with pMSC did not improve glycemia and showed increased pericapsular fibrosis. We conclude that pMSC derived from juvenile porcine exocrine pancreas improves insulin secretion of ICC by direct cell-cell contact. For transplantation purposes, the use of pMSC to support beta-cell function will depend on the development of new anti-fibrotic polymers and/or on genetically modified pigs with lower immunogenicity.
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