Journal
WATER RESEARCH
Volume 190, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2020.116737
Keywords
Anaerobic methane oxidation; Nitrate; Nitrite; Nitrogen elimination; Intertidal sediment; Yangtze Estuary
Funding
- National Natural Science Foundation of China [41601530, 41725002, 41971105, 41671463, 42030411, 41761144062, 41730646]
- Chinese National Key Programs for Fundamental Research and Development [2016YFA0600904, 2016YFA0600903, 2016YFE0133700]
- Yangtze Delta Estuarine Wetland Station, East China Normal University
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The study revealed the distribution pattern and controlling factors of nitrite/nitrate-dependent anaerobic methane oxidation (n-DAMO) bioprocesses in estuarine and intertidal marshes. It showed that Candidatus 'Methylomirabilis oxyfera'-like DAMO bacteria and Candidatus 'Methanoperedens nitroreducens'-like DAMO archaea cooccurred, with higher abundance of the M. oxyfera-like bacteria and a positive correlation with sediment pH and ammonium content.
Nitrite/nitrate-dependent anaerobic methane oxidation (n-DAMO) is a crucial link between carbon and nitrogen cycles in estuarine and coastal ecosystems. However, the factors that affect the heterogeneous variability in n-DAMO microbial abundance and activity across estuarine and intertidal wetlands remain unclear. This study examined the spatiotemporal variations in n-DAMO microbial abundance and associated activity in different estuarine and intertidal habitats via quantitative PCR and C-13 stable isotope experiments. The results showed that Candidatus 'Methylomirabilis oxyfera' (M. oxyfera)-like DAMO bacteria and Candidatus 'Methanoperedens nitroreducens' (M. nitroreducens)-like DAMO archaea cooccurred in estuarine and intertidal wetlands, with a relatively higher abundance of the M. oxyfera-like bacterial pmoA gene (4.0 x 10(6)-7.6 x 10(7) copies g(-1) dry sediment) than the M. nitroreducens-like archaeal mcrA gene (4.5 x 10(5) -9.4 x 10(7) copies g(-1) dry sediment). The abundance of the M. oxyfera-like bacterial pmoA gene was closely associated with sediment pH and ammonium (P<0.05), while no significant relationship was detected between M. nitroreducens-like archaeal mcrA gene abundance and the measured environmental parameters (P>0.05). High n-DAMO microbial activity was observed, which varied between 0.2 and 84.3 nmol (CO2)-C-13 g(-1) dry sediment day(-)1 for nitrite-DAMO bacteria and between 0.4 and 32.6 nmol (CO2)-C-13 g(-1) dry sediment day(-1) for nitrate-DAMO archaea. The total n-DAMO potential tended to be higher in the warm season and in the upstream freshwater and low-salinity estuarine habitats and was significantly related to sediment pH, total organic carbon, Fe(II), and Fe(III) contents (P<0.05). In addition to acting as an important methane (CH4) sink, n-DAMO microbes had the potential to consume a substantial amount of reactive N in estuarine and intertidal environments, with estimated nitrogen elimination rates of 0.5-224.7 nmol N g(-1) dry sediment day(-1). Overall, our investigation reveals the distribution pattern and controlling factors of n-DAMO bioprocesses in estuarine and intertidal marshes and gains a better understanding of the coupling mechanisms between carbon and nitrogen cycles. (c) 2020 Elsevier Ltd. All rights reserved.
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