Journal
VIRUS RESEARCH
Volume 298, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.virusres.2021.198385
Keywords
Porcine circovirus type 2; Cellular inflammatory responses; miR-21; circPDCD4; PDCD4; Porcine kidney 15 cell
Categories
Funding
- China Agriculture Research System [CARS-35]
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The study reveals that PCV2 infection leads to aberrant expression of miR-21 and PDCD4 in PK-15 cells, subsequently activating the NF-κB pathway and inflammatory responses.
Porcine circovirus type 2 (PCV2) causes postweaning multisystemic wasting syndrome (PMWS) as well as other PCV-associated diseases (PCVADs), which seriously affect the development of the swine industry. The association between aberrant expression of microRNA-21 (miR-21) and the pathogenesis of inflammatory diseases is already known. Also, our previous study showed elevated ssc-miR-21-5p (miR-21) levels in PCV2-infected porcine kidney 15 (PK-15) cells. However, the functional expression of miR-21 in PCV2 infection is unknown. Here, we found that the miR-21 levels were substantially upregulated in PCV2-infected cells, while the expression of PDCD4 and a PDCD4-derived circRNA (circPDCD4) were downregulated compared with the noninfected cells. The results of RNA immunoprecipitation and dual-luciferase reporter (DLR) assay revealed that circPDCD4 acted as a miR-21 sponge and PDCD4 was a miR-21 target. Functionally, we found that miR-21 overexpression induced the NF-?B pathway along with inflammation in cells exposed to PCV2. Further, the overexpression of PDCD4 or circPDCD4 downregulated miR-21 levels and subsequently, attenuated the miR-21-induced activation of inflammation and the NF-?B pathway. Thus, PCV2 activated the NF-?B pathway and cellular inflammatory responses through regulating circPDCD4, miR-21, and PDCD4 in PK-15 cells.
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