Journal
TALANTA
Volume 223, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2020.121758
Keywords
Cysteine detection; NIR fluroescence Probe; Large Stokes shift; Living cells imaging; Thionoesters; H2S donors
Categories
Funding
- National Natural Science Foundation of China [81801750, 81771517, 31802245]
- Key Project of Science and Technology of Henan Province [202102310473, 192102310235]
- doctor foundation of Xinxiang Medical University [XYBSKZZ201637]
- Science Foundation of Basic Medical School of Xinxiang Medical University [JCYXYKY201906]
- Key Scientific Research Foundation of the Higher Education Institutions of Henan Province, China [21B350002, 19A150006]
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A novel near-infrared (NIR) probe, termed TMN-Cys, has been constructed in this study, which can selectively detect cysteine in solution and has been successfully applied in monitoring endogenous Cys in living cells and mice.
Cysteine (Cys) as a vital antioxidant molecule and an effective biomarker for illness, plays an essential role in physiological functions and pathological processes. Extensive work has been done to explore the physiological functions of Cys and develop probes for detection of biothiols. However, the challenge to differentiate Cys from glutathione and homocystine remains. In this work, we constructed a novel near-infrared (NIR) probe, termed TMN-Cys, using TMN-NH2 and thionoesters. The probe could selectively detect Cys over homocysteine and glutathione in solution. It displayed a large Stokes shift (210 nm) upon treatment with Cys, and its detection limit was as low as 79 nM. Moreover, this probe showed low toxicity and was successfully employed in monitoring endogenous Cys in living cells and mice.
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