Journal
TALANTA
Volume 223, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2020.121779
Keywords
Fluorescent probe; Ratiometric; Hydrazine; Cells imaging; Sp20 myeloma cells
Categories
Funding
- National Natural Science Foundation of China [51678409, 51638011, 51578375, 81801808]
- Tianjin Research Program of Application Foundation and Advanced Technology of china [19JCYBJC19800, 18JCYBJC87500, 15ZCZDSF00880]
- State Key Laboratory of Separation Membranes and Membrane Processes [Z1-201507]
- Program for Innovative Research Team in University of Tianjin [TD13-5042]
- Harbin Medical University Cancer Hospital Haiyan Foundation [JJZD2018-02]
Ask authors/readers for more resources
A novel ratiometric fluorescent probe based on the fluorescein-coumarin structure was designed and synthesized for detecting hydrazine. The probe showed linear detection ranges and low LOD, indicating high sensitivity and selectivity for monitoring hydrazine in cells. It has great potential for application in detecting and cell imaging of hydrazine in actual water samples.
According to the mechanism of hydrazine to ester bond elimination, a novel ratiometric fluorescent probe (FCP) based on the fluorescein-coumarin structure is designed and synthesized for detecting hydrazine. The obvious red shift in the absorption and fluorescence spectrum is caused by the hydrolysis of the ester bond of FCP by upon mixing with hydrazine. The proposed FCP probe is shown to have linear detection ranges from 0 to 250 nM for hydrazine, and LOD is 0.364 nM. In addition, this promising probe possesses high sensitivity and selectivity for monitoring the intracellular hydrazine, thus, has great potential to be applied in early diagnosis of disease. Meanwhile, it has been successfully applied to the detection and cell imaging of hydrazine in actual water samples.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
