Gold nanoparticles capped with polysaccharides extracted from pineapple gum: Evaluation of their hemocompatibility and electrochemical sensing properties

In the present work, gold nanoparticles were synthesized through a green route by using, for the first time, polysaccharides extracted from pineapple gum (PG) as the reducing and capping agent. The obtained nanoparticles (AuNPs-PG) were characterized by UV-VIS, FTIR, TEM, FESEM, EDX, XRD, and zeta potential measurements, which confirmed that PG was effective to produce AuNPs with an average diameter of 10.3 +/- 1.6 nm. The AuNPs-PG were employed as the modifier of glassy carbon paste electrodes (CPE/AuNPs-PG), which were applied as sensitive electrochemical sensors to the determination of the antihistamine drug promethazine hydrochloride (PMZ). Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) measurements showed that the AuNPs-PG could enhance the electronic transfer properties of the glassy carbon paste, which was due to their large surface area and high electrical conductivity. After optimization of the instrumental parameters of square wave voltammetry (SWV) through a Box-Behnken factorial design, a linear relationship between the anodic peak current and PMZ concentration was obtained in the range from 2.0 to 15.7 mu mol L-1 in McIlvaine buffer solution pH 5.0. The detection and quantification limits were found to be equal to 1.33 and 4.44 mu mol L-1, respectively. The developed sensors could successfully quantify PMZ in different commercial pharmaceutical formulations, with satisfactory levels of accuracy and precision. In addition to improving the analytical features of the electrodes, hemocompatibility assays carried out on erythrocytes and leukocytes showed that the AuNPsPG do not exhibit toxic effects on the referred cells. This interesting behavior enables their use in biocompatible electrochemical sensing platforms as well as for future biomedical investigations.

Automated summary

In this study, gold nanoparticles were synthesized using polysaccharides extracted from pineapple gum, which showed effective results in sensitive electrochemical sensing of the antihistamine drug promethazine hydrochloride. The nanoparticles demonstrated good analytical features and biocompatibility for potential biomedical applications.