Quantification of 13C, 15N labelled compounds with 13C, 15N edited 1H Nuclear Magnetic Resonance spectroscopy

It was significant to detect isotope labelled compounds in biology and pharmacy. Based on a novel H-1 Nuclear Magnetic Resonance (H-1-NMR) technique, a simple, fast and green method has been successfully established to quantitatively detect C-13, N-15 isotope labelled compounds. In this protocol, the couples between H-1 and C-13, N-15 nearby were removed, which greatly simplified the spectrum. At mean time, the multiple peaks led by C-13 and N-15 were combined into one peak, so the signal intensity was also significantly enhanced. Melamine was selected as the internal standard and five C-13, N-15 isotope labelled compounds showed excellent linearity from 0.001 mM to 100 mM. A real polypeptide sample has quantitatively been detected.

Automated summary

A simple, fast, and green method has been established to quantitatively detect isotope labelled compounds in biology and pharmacy using a novel H-1 Nuclear Magnetic Resonance (H-1-NMR) technique. This method removes the coupling between nearby H-1 and C-13, N-15, simplifying the spectrum and enhancing the signal intensity, allowing for the quantitative detection of real polypeptide samples.