Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 249, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2020.119299
Keywords
Hydrogen sulfide; Copper (II) ions; Dual-imaging; Fluorescent probe; Living cells; Zebrafish
Categories
Funding
- National Natural Science Foundation of China [21502067, 31971605]
- National Key R&D Program of China [2017YFB0308000]
- Foundation of State Key Laboratory of Biobased material and Green papermaking [ZZ20200117]
- Qilu University of Technology [81110561]
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Development of molecular tools to track metal ions and active small molecules in living cells is crucial. The fluorescent probe (D-CN) can offer fluorescence imaging of H2S and copper (II) ions with different response signals. It was found that the cytotoxicity of copper ions in living cells increases in the presence of hydrogen sulfide.
Multiple types of metal ions and active small molecules (reactive nitrogen species, reactive oxygen species, reactive sulfur species, etc.) exist in living organisms. They have connections to each other and can interact and/or interfere with each other. To investigate the relationship of metal ions and active small molecules in living cells, it is necessary and critical to develop molecular tools that can track two kinds of associated certain metal ions and reactive molecules with multiple fluorescence signals. However, this is a challenging task that requires an ingenious molecular design to achieve this goal. Here, we present a fluorescent probe (D-CN) that can offer fluorescence imaging of H2S and copper (II) ions with different response signals. Recognition of H2S and Cu (II) by the new probe can result in green and red emissions, respectively, providing different signal responses to the two substances in living cells and zebrafish. In addition, we used this probe to visually prove that the cytotoxicity of copper ions in living cells increases in the presence of hydrogen sulfide and could lead to cell apoptosis. (C) 2020 Elsevier B.V. All rights reserved.
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