Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 336, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2021.129597
Keywords
SERS; IL-6; Serum; 2D substrate
Funding
- National Natural Science Foundation of China [61575043, 11874006]
- Natural Science Foundation of Fujian Province of China [2016J01292, 2018J01786]
- Fujian Provincial Department of Science and Technology guiding Project [2020Y0019]
- Industry-university Cooperation Project of Fujian Provincial Department of Science and Technology [2020N5006]
- Achievement Transformation Project of Fuzhou Science and Technology Bureau [2020-GX-20]
- Fushimei agricultural and rural maker space [2]
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A two-dimensional surface enhanced Raman spectroscopy (SERS) substrate was successfully prepared for trace detection of Interleukin-6 (IL-6) in plasma, by capturing glycoproteins in serum and using nanoprobes modified with IL-6 antibody. The method can achieve quantitative detection of IL-6 with a detection limit of 5 pg/mL and an applicable range of 0.5-50000 pg/mL.
We successfully prepared a two-dimensional surface enhanced Raman spectroscopy(SERS) substrate for the trace detection of Interleukin-6(IL-6) in plasma. Glycoproteins in serum were captured by a two-dimensional silver plate modified with 4-mercaptobenzoic acid (4-MPBA). And then, the Au@Au core-shell nanoprobes modified with IL-6 antibody accurately recognized IL-6 glycoprotein. The Au@Au NPs were embedded with Prussian Blue (PB) molecule, which only peaked at 2172 cm-1 in the biological silence zone. The calibration model of 4-MPBA and PB double signal molecules can avoid the interference of other glycoprotein macromolecules in the environment, thus realizing the quantitative detection of IL-6. The results showed that the higher the IL-6 content, the stronger the Raman signal. And the detection limit of IL-6 in serum can reach 5 pg/mL, the applicable range is 0.5?50000 pg/mL (R2 = 0.9928). Therefore, this method can be used as a multifunctional SERS activity technology, which can be used to detect IL-6 ultra-sensitively and quantitatively.
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