Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 330, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.129335
Keywords
DNA frame; Reversible assembly and disassembly; Logic gate; Ratiometric; Sensing; Imaging
Funding
- National Natural Science Foundation of China [91859123]
- Fundamental Research Funds for Central University, Nankai University [63201043]
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By controlling the assembly/disassembly of DNA tetrahedron, three binary INHIBIT logic gates were constructed, and various sensing systems were successfully developed for the ratiometric detection of microRNA, Hg2+, cysteine and pH value. The proposed sensing systems demonstrated efficient internalization of DNA nanostructures by cells for ratiometric imaging of microRNA, Hg2+ and pH in living cells.
Molecular computing holds great promise for intelligent diagnosis and treatment of diseases at molecular level, thus designing molecular logic gates to operate pmgrammably and autonomously for molecular analysis is urgently needed. In this work, controllable assembly/disassembly of DNA tetrahedron, a typical DNA frame structure, was achieved by alternate addition of two stimulating factors including nucleic acid and antisense nucleic acid, Hg2+ and cysteine, or H+ and OH-. Such a DNA frame structure assembly/disassembly behavior had been proved to be reversible and could be cycled for several times, which was clearly reflected by repeated switch-on and switch-off of the fluorescence resonance energy transfer signal. Based on this principle, three binary INHIBIT logic gates were constructed by using different input combinations. Such a principle was also successfully used to develop various sensing systems for the ratiometric detection of microRNA, Hg2+, cysteine and pH. Thanks to the highly efficient internalization of DNA nanostructures by cells, the proposed sensing systems were demonstrated to work well for the ratiometric imaging of microRNA, Hg2+ and pH value in living cells.
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