4.7 Article

Amplification-free electrochemiluminescence molecular beacon-based microRNA sensing using a mobile phone for detection

Journal

SENSORS AND ACTUATORS B-CHEMICAL
Volume 330, Issue -, Pages -

Publisher

ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.129261

Keywords

Electrogenerated chemiluminescence; Electrochemiluminescence; microRNA; Biosensor; Point-of-care biosensor; Mobile phone microRNA detection

Funding

  1. National Health and Medical Research Council (NHMRC) of Australia [GNT1161573]
  2. CSIRO Probing Biosystems Future Science Platform
  3. Australian Research Council [DP200102947]
  4. Australian Research Council [DP200102947] Funding Source: Australian Research Council

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The sensor demonstrated in this study combines an ECL molecular beacon with magnetic bead based extraction of the miRNA target sequence to provide a sensitive and portable solution for miRNA-21 detection. The sensor does not require intercalating labels, reporter probes, added enzymes or hairpin primers for target amplification, offering a simple and robust strategy for miRNA detection.
microRNAs (miRNAs) have emerged as important biomarkers for numerous diseases, but their widespread use has been hampered by the lack of point-of-care testing solutions. Herein, we demonstrate a sensitive, rapid and portable electrochemiluminescence (ECL) based sensor for miRNA-21 combining a switch-on ECL molecular beacon with magnetic bead based extraction of the miRNA target sequence. Streptavidin coated magnetic beads were functionalised with a hairpin molecular beacon; one stem of the beacon was functionalised with an ECL active label and the opposing stem was functionalised with a quencher. When the target miRNA was present, the hairpin opened, separating the label and quencher, generating an intense ECL signal. The sensor provides a simple and robust strategy for miRNA detection without the need for intercalating labels, reporter probes for detection, added enzymes or hairpin primers for target amplification. The detection limit of the sensor is 500 attomoles of miRNA-21 when using a photomultiplier tube for ECL detection. The potential of the developed sensor for point-of-care applications has been demonstrated by combining the molecular beacon ECL sensor with a portable potentiostat for ECL generation and a mobile phone camera for ECL detection of miRNA-21.

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