High throughput DNA concentration determination system based on fluorescence technology

The measurement of DNA concentration is an important step in many research procedures in molecular biology. Traditional method for DNA concentration determination is based on ultraviolet absorbance which suffers from low sensitivity. Thus, we proposed a high throughput and low-cost DNA concentration system based on fluorescence technology. We analyzed the self-luminescence of each individual solution in DNA test kit, such as fluorescence dyes and DNA. It showed that the fluorescence emission from the dsDNA/dye complex was significantly higher than the self-luminescence from the single SYBR Green I, Gel Green and DNA solution when it was excited by laser diode source. SYBR Green I and Gel Green preferred CG bp sequences in dsDNA. dsDNA ranged from 2 to 15 ng/mu L presented a stronger linear relationship with relative fluorescence intensity (R = 0.995). Such a low cost system could measure the concentration of 24 DNA samples within 100 s. It permits the estimation of concentrations of DNA as low as 2 ng/mu L and offers a simple way for DNA concentration determination during biological research, especially for the labs in the third world countries.

Automated summary

The researchers proposed a high throughput and low-cost DNA concentration system based on fluorescence technology, which can measure the concentration of 24 DNA samples within 100 seconds by analyzing the self-luminescence of individual solutions in DNA test kit. This system provides a simple way for DNA concentration determination and is suitable for labs in third world countries.