Journal
SENSORS
Volume 21, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/s21041223
Keywords
antibody; immunoassay; fluorescence; quench; detection; biomarker
Funding
- National Natural Science Foundation of China [21775064]
- Natural Science Foundation of Shandong Province [ZR2017MB037]
- JSPS KAKENHI from the Japan Society for the Promotion of Science, Japan [JP18H03851]
- Japan Science and Technology Agency, Japan [JPMJMI18D9]
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The detection of viruses, disease biomarkers, physiologically active substances, drugs, and chemicals is important in many areas of life, and immunodetection technology offers advantages of simplicity and good selectivity. Quenchbody, as a new type of fluorescent immunosensor, can detect antigens through changes in fluorescence intensity, providing a simple, easy to operate, and highly sensitive method of detection.
The detection of viruses, disease biomarkers, physiologically active substances, drugs, and chemicals is of great significance in many areas of our lives. Immunodetection technology is based on the specificity and affinity of antigen-antibody reactions. Compared with other analytical methods such as liquid chromatography coupled with mass spectrometry, which requires a large and expensive instrument, immunodetection has the advantages of simplicity and good selectivity and is thus widely used in disease diagnosis and food/environmental monitoring. Quenchbody (Q-body), a new type of fluorescent immunosensor, is an antibody fragment labeled with fluorescent dyes. When the Q-body binds to its antigen, the fluorescence intensity increases. The detection of antigens by changes in fluorescence intensity is simple, easy to operate, and highly sensitive. This review comprehensively discusses the principle, construction, application, and current progress related to Q-bodies.
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