Influence of extracellular polymeric substance on the interaction between titanium dioxide nanoparticles and Chlorella pyrenoidosa cells

The presence of extracellular polymeric substance (EPS) plays a vital role in the accumulation and toxicity of nanoparticles to microorganisms, in which the involved processes and mechanisms are still waiting to be revealed. Herein, we specifically investigated the interfacial interaction between titanium dioxide nanoparticles (nTiO(2)) and algae (Chlorella pyrenoidosa) with/without EPS and the effect of EPS on algal cell internalization of nTiO(2). Results showed that the presence of EPS on cell surface promoted heteroaggregation between nTiO(2) and algal cells, and induced more nTiO(2) accumulation on algal surface; however, algal cell internalization of nTiO(2) was limited by the presence of EPS. Pearson correlation analysis further proved that the presence of EPS had a positive effect on the surface accumulation of nTiO(2) and a negative effect on the internalization of nTiO(2). More than 60% of cell internalized nTiO(2) entered algal cells through the energy dependent endocytosis pathway. It is interesting to find that anatase nTiO(2) (nTiO(2)-A) entered algal cells mainly through the clathrin dependent endocytosis, while rutile nTiO(2) (nTiO(2)-R) mainly through the dynamin dependent endocytosis. This difference could be due to the different affinities of nTiO(2)-A and nTiO(2)-R to the mediating receptors referring to different endocytic pathways. The removal of EPS activated the associated mediating pathways, allowing more nTiO(2) to be internalized. These findings address the role of EPS on the interaction between nTiO(2) and algae and promote a deeper understanding of the ecological effect of nTiO(2). (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

The presence of extracellular polymeric substance (EPS) affects the interfacial interaction between titanium dioxide nanoparticles (nTiO(2)) and algae, promoting surface accumulation of nanoparticles on algal cells while limiting their internalization. The different endocytosis pathways for anatase nTiO(2) and rutile nTiO(2) entering algal cells may be attributed to their affinities to mediating receptors. EPS removal activates mediating pathways, facilitating more nanoparticle internalization.