Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 760, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.scitotenv.2020.144040
Keywords
Antibiotic resistance genes; Horizontal transfer; Nano-TiO2 photoexcitation; Phage transduction; Reactive oxygen species (ROS)
Categories
Funding
- National Key Research and Development Project of China [2020YFC1808204]
- National Natural Science Foundation of China [51878317, 21590812, 51821006]
- Recruitment Program for Leading Talent Team of Anhui Province, China [2019-16]
Ask authors/readers for more resources
This study found that TiO2 photoexcitation has a synergistic promotion effect on phage infection, but excessive UV irradiation may result in a decrease in transductant formation. ROS produced by moderate TiO2 photoexcitation facilitate the phage infection process.
Environmental pollution caused by antibiotic resistance genes (ARGs) has attracted wide concerns, and various approaches have been proposed to control ARGs dissemination. TiO2 photoexcitation under UV irradiation has been used for such a purpose. But the actual UV intensity is insufficient to trigger the production of reactive oxygen species (ROS) in the aqueous environment. Thus, it is interesting to know how mild photoexcitation of TiO2 with low-intensity UV affects the horizontal transfer of ARGs. In this work, the impact of TiO2 photoexcitation on the transductant efficiency of constructed filamentous phage gM13 to its host Escherichia coli TG1 was investigated. Although individual treatment with nano-TiO2 and UV irradiation both improved the phage infection, TiO2 photoexcitation exhibited a clear synergistic promotion effect. However, excessive UV irradiation resulted in a decrease in transductant formation, implying severe oxidative damage to the phage and bacterial cells. Extracellular ROS produced by moderate photoexcitation of TiO2 could increase the outermembrane permeability, which facilitated phage infection. The increase in pili synthesis induced by intracellular ROS provided more sites for phage recognition and invasion in the presence of TiO2 photoexcitation, which contributed to the transduction process. Our work provides a novel insight into the impact of TiO2 photoexcitation on ARGs diffusion and is helpful for better understanding non-toxic environmental effect of nanomaterials. (C) 2020 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available