Interrogating lncRNA functions via CRISPR/Cas systems

Long noncoding RNAs (lncRNAs) are an increasing focus of investigation due to their implications in diverse biological processes and disease. Nevertheless, the majority of lncRNAs are low in abundance and poorly conserved, posing challenges to functional studies. The CRISPR/Cas system, an innovative technology that has emerged over the last decade, can be utilized to further understand lncRNA function. The system targets specific DNA and/or RNA sequences via a guide RNA (gRNA) and Cas nuclease complex. We and others have utilized this technology in various applications such as lncRNA knockout, knockdown, overexpression, and imaging. In this review, we summarize how the CRISPR/Cas technology provides new tools to investigate the roles and therapeutic implications of lncRNAs.

Automated summary

Long noncoding RNAs (lncRNAs) have become a focus of investigation due to their roles in various biological processes and diseases. The innovative CRISPR/Cas system technology offers new tools for studying lncRNA function, allowing applications such as knockout, knockdown, overexpression, and imaging. This technology has the potential to provide insights into the roles and therapeutic implications of lncRNAs.