4.2 Article

Permanent diaphragmatic deficits and spontaneous respiratory plasticity in a mouse model of incomplete cervical spinal cord injury

Journal

RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
Volume 284, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.resp.2020.103568

Keywords

Micee; C2 spinal cord injury; Diaphragm EMG; Phrenic motoneuron

Funding

  1. Chancellerie des Universites de Paris (Legs Poix)
  2. Fondation de France
  3. Fondation Medisite
  4. INSERM
  5. Universite de Versailles Saint-Quentin-en-Yvelines
  6. Ministry of Science and Technology [109-2636-B-110-001]

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The study reveals that there was no significant spontaneous recovery of diaphragmatic activity observed up to 30 days post-injury in a C57BL/6 mouse model of high spinal cord injuries, but a partial restoration of the injured diaphragm was observed at 7 days post-injury during a respiratory challenge. Additionally, there was an increase in phrenic motoneuronal excitability between 1-7 days post-injury on the injured side, reflecting plasticity in the mouse respiratory system following a C2 cervical hemisection.
High spinal cord injuries (SCI) lead to permanent respiratory insufficiency, and the search for new therapeutics to restore this function is essential. To date, the most documented preclinical model for high SCI is the rat cervical C2 hemisection. However, molecular studies with this SCI model are limited due to the poor availability of genetically modified specimens. The aim of this work was to evaluate the pathophysiology of respiratory activity following a cervical C2 injury at different times post-injury in a C57BL/6 mouse model. No significant spontaneous recovery of diaphragmatic activity was observed up to 30 days post-injury in eupneic condition. However, during a respiratory challenge, i.e. mild asphyxia, a partial restoration of the injured diaphragm was observed at 7 days post-injury, corresponding to the crossed phrenic phenomenon. Interestingly, the diaphragmatic recording between 2 respiratory bursts on the injured side showed an amplitude increase between 1-7 days postinjury, reflecting a change in phrenic motoneuronal excitability. This increase in inter-burst excitability returned to pre-injured values when the crossed phrenic phenomenon started to be effective at 7 days post-injury. Taken together, these results demonstrate the ability of the mouse respiratory system to express long-lasting plasticity following a C2 cervical hemisection and genetically modified animals can be used to study the pathophysiological effects on these plasticity phenomena.

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