Journal
RESEARCH IN VETERINARY SCIENCE
Volume 135, Issue -, Pages 200-216Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2021.02.013
Keywords
Cardiac conduction fibers; Gap junctions; Desmin; Connexin; PAS method
Categories
Funding
- Instituto de Salud Carlos III
- FEDER [PI14/00271, pie15/00013]
- Generalitat Valenciana [PROMETEO2013/007]
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This study conducted a morphometric analysis of cardiac conduction cells in male horse and dog hearts, revealing significant differences in density and thickness between the two species. Additionally, the study found that the area and diameter of cardiac conduction cells were larger in horses than in dogs. Dogs also had a higher number of junctions compared to horses.
The principal function of the ventricular conduction system is rapid electrical activation of the ventricles. The aim of this study is to conduct a morphometric study to pinpoint the morphological parameters that define cardiac conduction cells, allowing us to distinguish them from other cells. Five male horse hearts and five male dog hearts were used in the study. The hearts were fixed in a 5% formaldehyde solution. Histological sections of 5 ?m thickness were acquired and stained with hematoxylineosin and Masson?s trichrome and cardiac conduction cells and their junctions were identified by desmin, connexin 40 and a PAS method. We found statistically significant differences in cardiac conduction fibers density and thickness, which was much higher in horses than in dogs (p = 0.000 for both values). By comparing the measured parameters of the cells in both species, we determined that cardiac conduction cells area and diameters were greater in horses than in dogs (p = 0.000 for all values). In dogs there are more junctions (30.8%) than in horses (26.1%), a statistically significant difference (p = 0.041). Our findings regarding the cardiac conduction fibers distribution in the animal species studied becomes new knowledge that contributes to the morphological study of this component of the cardiac conduction system and also makes it possible to locate exactly the site with the highest density of cardiac conduction fibers as a contribution to the cardiological study of these structures that lead to the prevention of ventricular arrhythmias and the identification of their treatment site.
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