Journal
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
Volume 132, Issue -, Pages 53-65Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.envexpbot.2016.08.007
Keywords
Abiotic stress; Biotic stress; Chitinase; Co-Immunoprecipitation; Osmotin like protein; Pathogenesis-related proteins; Phosphofructokinase2
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Funding
- UGC, New Delhi [32-569/2006 (SR)]
- CSIR, New Delhi
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Osmotin like protein (OLP) and chitinase (Chi11) belong to pathogenesis-related (PR) class of proteins and are induced during both biotic and abiotic stresses. Transgenic tomato was developed with OLP and Chi11 via in vitro and in planta transformation methods. Transgenes integration and transcript levels were confirmed by multiplex PCR, DNA blot, and multiplex reverse transcriptase PCR. Homozygous T-2 transgenics when evaluated for salt, drought, and fungal stresses showed enhanced tolerance compared to untransformed controls (UC). Transgenics showed enhanced endochitinase activity and root biomass under normal conditions. Transformants also displayed higher proline content, K+, relative water content, chlorophyll fluorescence, total biomass, vascular conductivity, and fruit yield than the UC under stress conditions. Co-immunoprecipitation revealed that Chi11 co-expresses with phosphofructokinase2 (PFK2), which may play a role in enhanced root biomass. qPCR analysis resulted in higher transcript levels of OLP, Chi11, and PFK2 in transgenics as compared to the untransformed controls. Our findings suggest the use of multiples genes to confer multiple stress tolerance for enhanced crop productivity. This work unveils a new molecular player PFK2, which may play a role in enhanced root biomass. (C) 2016 Elsevier B.V. All rights reserved.
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