Journal
PROCESS BIOCHEMISTRY
Volume 102, Issue -, Pages 33-41Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2020.11.029
Keywords
VK2 (MK-7); Bacillus velezensis; Fermentation; qRT-PCR
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Funding
- National Natural Science Foundation of China [21366028]
- China Postdoctoral Science Foundation [2017M613301XB]
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This study isolated and characterized a novel bacterial strain Bacillus velezensis ND capable of producing MK-7. Low-cost substrates were utilized to reduce the production cost, with the highest MK-7 yield achieved through solid-state fermentation. Key genes involved in the MK synthesis pathway were found to be positively correlated with MK-7 biosynthesis under different fermentation processes.
In this study, a novel MK-7 producing bacterial strain, Bacillus velezensis ND, was isolated and characterized. Multiple low-cost substrates were used in Bacillus velezensis ND culture medium to reduce the MK-7 production cost, MK-7 concentration of 43.8 +/- 2.8 mg/L was obtained using raw soybean flour by liquid-state fermentation (LSF). To improve the MK-7 production, LSF, biofilm-based fermentation (BF), and the solid-state fermentation (SSF) were compared. The highest MK-7 yield was achieved by SSF is 150.0 +/- 4.9 mg/kg. The associated synthetic gene expressions under these three fermentation processes were investigated as well. The result indicated that the key genes menF, menH, and menG in the MK synthesis pathway are positively correlated with the increase of MK-7 biosynthesis.
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