Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 118, Issue 8, Pages -Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2007192118
Keywords
Trichoderma; homologous recombination; meiosis; Rad51; Dmc1
Categories
Funding
- Academia Sinica, Taiwan, Republic of China [AS-105-TP-B07, AS108-TP-B07]
- Ministry of Science and Technology, Taiwan, Republic of China [MOST 1632311-B-001-016-MY3, MOST 105-2314-B-002-073-MY4, MOST 107-2113-M-002-010-MY3]
Ask authors/readers for more resources
Most eukaryotes possess two RecA-like recombinases for interhomolog recombination during meiosis, but some have lost Dmc1. TrRad51 in Trichoderma reesei plays a crucial role in interhomolog recombination and has better mismatch tolerance than ScRad51, evolving ScDmc1-like properties through structural variations.
Most eukaryotes possess two RecA-like recombinases (ubiquitous Rad51 and meiosis-specific Dmc1) to promote interhomolog recombination during meiosis. However, some eukaryotes have lost Dmc1. Given that mammalian and yeast Saccharomyces cerevisiae (Sc) Dmc1 have been shown to stabilize recombination intermediates containing mismatches better than Rad51, we used the Pezizomycotina filamentous fungus Trichoderma reesei to address if and how Rad51-only eukaryotes conduct interhomolog recombination in zygotes with high sequence heterogeneity. We applied multidisciplinary approaches (next- and third-generation sequencing technology, genetics, cytology, bioinformatics, biochemistry, and single-molecule biophysics) to show that T. reesei Rad51 (TrRad51) is indispensable for interhomolog recombination during meiosis and, like ScDmc1, TrRad51 possesses better mismatch tolerance than ScRad51 during homologous recombination. Our results also indicate that the ancestral TrRad51 evolved to acquire ScDmc1-like properties by creating multiple structural variations, including via amino acid residues in the L1 and L2 DNA-binding loops.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available