Journal
ENVIRONMENT INTERNATIONAL
Volume 89-90, Issue -, Pages 30-37Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.envint.2016.01.008
Keywords
Housedust; Primary human corneal epithelial cells; Oxidative stress; Inflammation; Mitochondrial dysfunction
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Funding
- Jiangsu Double-Innovation Program, Jiangsu Provincial Innovation Fund [0211001802]
- Graduate Student Innovation Project of Jiangsu Province [KYZZ15_0034]
- National Natural Science Foundation of China [21577055]
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Human cornea is highly susceptible to damage by dust Continued daily exposure to housedust has been associated with increasing risks of corneal injury, however, the underlying mechanism has not been elucidated. In this study, a composite housedust sample was tested for its cytotoxicity on primary human corneal epithelial (PHCE) cells, which were exposed to dust at 5-320 mu g/100 mu L for 24 h. PHCE cell viability showed a concentration-dependent toxic effect, attributing to elevated intracellular ROS. Moreover, when exposed at >20-80 mu g/100 mu L, dust-induced oxidative damage was evidenced by increased malondialdehyde and 8-hydroxy-2-deoxyguanosine (1.3-2.3-fold) and decreased antioxidative capacity (1.6-3.5-fold). Alteration of mRNA expression of antioxidant enzymes (SOD1, CAT, HO-1, TRXR1, GSTM1, GSTP1, and GPX1) and pro-inflammatory mediators (IL-1 beta, IL-6, IL-8, TNF-alpha, and MCP-1) were also observed. Furthermore, the mitochondrial transmembrane potential was dissipated from 9.2 to 82%. Our results suggested that dust-induced oxidative stress probably played a vital role in the cytotoxicity in PHCE cells, which may have contributed to dust-induced impairment of human cornea. Published by Elsevier Ltd
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