4.7 Article

Rapid detection of H146-like goose calicivirus using a TaqMan-based real-time PCR assay

Journal

POULTRY SCIENCE
Volume 100, Issue 2, Pages 482-487

Publisher

ELSEVIER
DOI: 10.1016/j.psj.2020.11.016

Keywords

goose calicivirus; H146-like; real-time PCR; TaqMan probe; virus detection

Funding

  1. Fujian Natural Science Foundation, China [2019R1026-3]

Ask authors/readers for more resources

A novel TaqMan-based real-time PCR approach was developed to reliably detect and diagnose H146-like GCV, enabling real-time diagnosis of infections and providing a greater understanding of the epidemiology and clinical presentation of the disease.
H146-like goose-origin calicivirus (H146-like GCV) is a novel Caliciviridae family member in the Sanovirus genus that was recently discovered and proposed to cause runting-stunting syndrome and urate deposition in geese. At present, however, there is a lack of epidemiological information pertaining to the dynamics and distribution of H146-like GCV. The development of novel molecular diagnostic approaches capable of rapidly and accurately detecting this virus would support the strengthening, the prevention, and control of H146-like GCV infection. In the present study, we therefore used a TaqMan probe and primers specific for the viral nonstructural (NS) gene to develop a highly sensitive and specific PCR assay capable of detecting this H146-like GCV. The assay reproducibly detected 5.07 x 10(2) copies of a recombinant DNA plasmid containing the NS gene, with a dynamic range of 8 orders of magnitude (10(2)-10(9) copies). Importantly, no cross-reactivity was observed with common viruses that affected waterfowl, and when we used this assay to evaluate clinical samples, we found it to be more sensitive and faster than traditional PCR. In summary, herein, we developed a novel TaqMan-based real-time PCR approach that could reliably detect and diagnose H146-like GCV. This tool will allow for the real-time diagnosis of H146-like GCV infections, enabling researchers to better understand the epidemiology and clinical presentation of this disease.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available