Journal
POLYMER JOURNAL
Volume 53, Issue 6, Pages 741-746Publisher
SPRINGERNATURE
DOI: 10.1038/s41428-021-00464-4
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Funding
- Takeda Science Foundation
- KOSE cosmetology research foundation
- JSPS Research Fellowships for Young Scientists
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In this study, a reduction-responsive oligonucleotide was constructed by post-modifying an oligonucleotide with a diazo compound bearing a 4-nitrobenzyl group. The introduction of the 4-nitrobenzyl group and the subsequent reduction-triggered recovery of the original oligonucleotide were confirmed using HPLC and mass spectrometry. The protocol for preparing this reduction-responsive oligonucleotide is simple and it holds promise for various applications in the fields of chemical and synthetic biology.
Herein, we describe the construction of a reduction-responsive oligonucleotide by post-modification of an oligonucleotide with a diazo compound bearing a 4-nitrobenzyl group as a reduction-responsive cleavable moiety. High-performance liquid chromatography and mass spectrometry were used to reveal the introduction of a 4-nitrobenzyl group to the 5'-phosphate group of an oligonucleotide, and the subsequent reduction-triggered recovery of the original oligonucleotide. The protocol used for the preparation of this reduction-responsive oligonucleotide is simple and it will have various applications in the fields of chemical and synthetic biology.
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