Nutrient stoichiometry of a plant-microbe-soil system in response to cover crop species and soil type

Aims The theory of ecological stoichiometry mostly builds on studies of natural terrestrial ecosystems, whereas only limited stoichiometry information is available in response to agronomic practices. Methods We designed a greenhouse experiment in order to disentangle the specific role of cover crop identity and soil characteristic in affecting nutrient stoichiometry of a plant-microbe-soil system. Results Nutrient ratios of cover crop biomass were species-specific and the growth rate explained, for most species considered, the stoichiometric differences in response to soil type. In contrast, the nutrient stoichiometry of soil microbes was more homeostatic and did not respond to either cover crop identity or soil type. Compared to bare soil, the presence of cover crop enhanced microbial phosphorus immobilization in the clay-rich soil, whereas it promoted microbial carbon biomass and microbial nitrogen immobilization in the sandy-rich soil. A greater microbial cumulative respiration in clay soils, where a higher microbial biomass C at the beginning of the incubation was observed, suggested a major role of soil type, compared to cover crop identity, in affecting microbial metabolism. Conclusions By understanding the stoichiometric constraints in the plant-microbe-soil system, our findings can help to implement agro-ecological practices by selecting appropriate cover crop species in relation to soil type in order, for example, to avoid nutrient limitation due to microbial nutrient immobilization.

Automated summary

The study revealed that nutrient ratios of cover crop biomass are species-specific, while the nutrient stoichiometry of soil microbes is more stable and not affected by cover crop identity or soil type. Results from nutrient comparison experiments indicate that cover crops have an impact on soil type, while soil type plays a more significant role in microbial metabolism.