Journal
PHYTOTHERAPY RESEARCH
Volume 35, Issue 6, Pages 3337-3350Publisher
WILEY
DOI: 10.1002/ptr.7054
Keywords
acute myeloid leukemia; differentiation; isobavachalcone; mitochondrial apoptosis; ROS
Categories
Funding
- NSFC [82003980, 81973530]
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The study demonstrates that Isobavachalcone induces apoptosis and differentiation of acute myeloid leukemia cells through multiple pathways, with the ROS-mediated signaling pathway playing a key role.
Isobavachalcone (IBC) has been shown to induce apoptosis and differentiation of acute myeloid leukemia (AML) cells. However, the underlying molecular mechanisms are not fully understood. Herein, IBC exhibited significant inhibition on the cell viability, proliferation, and the colony formation ability of AML cells. Moreover, IBC induced mitochondrial apoptosis evidenced by reduced mitochondrial membrane potential, increased Bax level, decreased Bcl-2, Bcl-xL, and Mcl-1 levels, elevated cytochrome c level in the cytosol and increased cleavage of caspase-9, caspase-3, and PARP. Furthermore, IBC obviously promoted the differentiation of AML cells, accompanied by the increase of the phosphorylation of MEK and ERK and the C/EBP alpha expression as well as the C/EBP beta LAP/LIP isoform ratio, which was significantly reversed by U0126, a specific inhibitor of MEK. Notably, IBC enhanced the intracellular ROS level. More importantly, IBC-induced apoptosis and differentiation of HL-60 cells were significantly mitigated by NAC. In addition, IBC also exhibited an obvious anti-AML effect in NOD/SCID mice with the engraftment of HL-60 cells. Together, our study suggests that the ROS-medicated signaling pathway is highly involved in IBC-induced apoptosis and differentiation of AML cells.
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