Journal
PHYTOCHEMISTRY
Volume 183, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2020.112611
Keywords
Magnetic solid phase extraction; In situ derivatization; Oilseeds; Phytohormones; Ultra-high performance liquid; chromatography-tandem mass spectrometry
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Funding
- National Natural Science Foundation of China [90817101, 30670190, 31900387]
- Scientific Research Fund of Hunan Provincial Education Department [16K042, 18C0130]
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A magnetic solid phase extraction method integrated with in situ derivations was developed for the profiling of 12 phytohormones in a single rapeseed seed. Satisfactory methodological performance was achieved through parameter optimization, allowing for successful analysis of target phytohormones in different oilseeds samples.
Magnetic solid phase extraction integrated with in situ derivations for the profiling of 12 phytohormones in a single rapeseed seed was developed by using ultra-high performance liquid chromatography-tandem mass spectrometry. The Fe3O4@Ti3C2@beta-cyclodextrin nanoparticles were firstly synthesized and used as an adsorbent for the solid-phase extraction of phytohormones. The magnetic dispersive solid-phase extraction and in situ derivation by the addition of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide were ingeniously combined. This efficient pre-treatment method integrated the extraction, purification, and derivatization processes into one single step. Satisfactory methodological performance was achieved by optimization of the parameters. Linearities (R-2 > 0.9928) and recoveries (80.4 %-115.1%) at three spiked levels, as well as the low matrix effect (from -16.63% to 17.06%) and limits of detection (0.89-13.62 pg/mL) were obtained. The spatio-temporal profiling of target phytohormones in different tissues of rapeseed germination was investigated. This method was successfully employed for analyzing target phytohormones in different oilseeds samples.
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