Journal
PHYSIOLOGY & BEHAVIOR
Volume 229, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.physbeh.2020.113236
Keywords
cAMP-response element binding protein; Depression; Insulin-like growth factor I; Mitochondrial dysfunction; Obesity
Categories
Funding
- National Clinical Key Subject Construction Project of NHFPC Fund
- National Natural Science Fund [81471252, 81772060]
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This study confirmed that obesity induced by high-fat diet promoted depression-like behaviors in mice, and insulin-like growth factor-1 (IGF-1) alleviated these behaviors and mitochondrial dysfunction through the activation of CREB/PGC-1 alpha signal pathway.
Background: Some evidence suggests that depression is more common in obese patients. This fact gives us a hint that obesity might be a promoter of depression, though a conclusion can not be drawn. The aim of the study was: (1) to confirm whether obesity induced by high-fat diet (HFD) promotes depression-like behaviors in mice, (2) to explore the protective role of insulin-like growth factor-1 (IGF-1) in such behavioral disorder of the animals and (3) to reveal whether mitochondrial mechanism was involved in such protective effect of the reagent. Methods: C57BL/6 J mice were fed with HFD to establish a model of obesity. Then, the animals were separately or simultaneously treated with PEG-IGF-1, 666-15 (CREB blocker) and SR-18292 (PGC-1 alpha blocker). After that, depression-like behaviors were assessed using sucrose preference test and tail suspension test. In hippocampus, respiratory control ratio, ATP generation and red/green fluorescence ratio were adopted to reveal mitochondrial function. Also in hippocampus, expressions of p-CREB and PGC-1 alpha were measured using western blotting. Results: HFD mice showed depression-like behaviors compared with control mice. Such diet also caused mito-chondrial dysfunction and inhibition of CREB/PGC-1 alpha signal pathway in hippocampus of these animals. After PEG-IGF-1 intervention, all the abnormalities mentioned above can be partly reversed. After 666-15 or SR-18292 treatment, such protective effect of PEG-IGF-1 can be attenuated, and the mice suffered from the re-deterioration of behavioral and mitochondrial abnormalities in hippocampus. Conclusion: IGF-1 alleviated depression-like behaviors and mitochondrial dysfunction through the activation of CREB/PGC-1 alpha signal pathway in HFD mice.
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