Multispectral compressive fluorescence lifetime imaging microscopy with a SPAD array detector

Multispectral/hyperspectral fluorescence lifetime imaging microscopy (lambda FLIM) is a promising tool for studying functional and structural biological processes. The rich information content provided by a multidimensional dataset is often in contrast with the acquisition speed. In this work, we develop and experimentally demonstrate a wide-field lambda FLIM setup, based on a novel time-resolved 18 x 1 single-photon avalanche diode array detector working in a single-pixel camera scheme, which parallelizes the spectral detection, reducing measurement time. The proposed system, which implements a single-pixel camera with a compressive sensing scheme, represents an optimal microscopy framework towards the design of lambda FLIM setups. (C) 2021 Optical Society of America

Automated summary

Multispectral/hyperspectral fluorescence lifetime imaging microscopy (lambda FLIM) is a promising tool for studying biological processes. The development of a wide-field lambda FLIM setup with a novel single-photon avalanche diode array detector reduces measurement time by parallelizing spectral detection. The proposed system, incorporating a single-pixel camera with a compressive sensing scheme, represents an optimal microscopy framework for lambda FLIM setups.