Journal
NEW BIOTECHNOLOGY
Volume 63, Issue -, Pages 37-44Publisher
ELSEVIER
DOI: 10.1016/j.nbt.2021.03.001
Keywords
EasyMAP; 16S ribosomal DNA; Microbiome
Funding
- Ministry of Science and Technology, Taiwan [MOST 102-2320-B-002-015]
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With the advancement of technology, an easy-to-use online platform EasyMAP has been developed for comprehensive analysis of microbial 16S ribosomal DNA sequences, providing users with various analytical tools and gene function predictions.
As next-generation sequencing technology has become more advanced, research on microbial 16S ribosomal DNA sequences has developed rapidly. Sequencing of 16S ribosomal DNA allows the composition of bacteria and archaea in a sample to be obtained and many analytical tools related to 16S ribosomal DNA sequences have been proposed; however, most do not include a user-friendly platform with a graphical user interface. Here, a comprehensive and easy-to-use online platform, Easy Microbiome Analysis Platform (EasyMAP), has been developed for analysis of 16S ribosomal DNA sequencing data. EasyMAP integrates the QIIME2, LefSe, and PICRUSt pipelines and includes temporal profiling analysis. Users can perform quality checks, taxonomy differential abundance analysis, microbial gene function prediction and longitudinal analysis with step-by-step guidance. EasyMAP is a user-friendly tool for comprehensive analysis of 16S ribosomal DNA sequencing data. The web server and documentation are freely available at http://easymap.cgm.ntu.edu.tw/.
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