Journal
NATURE METHODS
Volume 18, Issue 4, Pages 406-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41592-021-01080-z
Keywords
-
Categories
Funding
- Waitt Foundation
- NCI CCSG [CA014195]
- NSF NeuroNex [2014862]
- National Institutes of Health (NIH) [R21 DC018237]
- NIH [F32 GM137580, T32GM007240]
- Wicklow AI in Medicine Research Initiative
- NSF [1707356]
- NSF NeuroNex Award [2014862]
- NIH/NIMH [2R56MH095980-06]
- Parkinson's Foundation [PF-JFA-1888]
- NIH/NIGMS [R35GM128823]
- Japan Society for the Promotion of Science KAKENHI [17H06311, 19H03336]
- AMED [JP20dm0207084]
- NVIDIA Corporation
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1707356] Funding Source: National Science Foundation
- Grants-in-Aid for Scientific Research [19H03336] Funding Source: KAKEN
Ask authors/readers for more resources
Point-scanning imaging systems are widely used for high-resolution cellular and tissue imaging, but optimizing resolution, speed, sample preservation, and signal-to-noise ratio simultaneously is challenging. The use of deep learning-based supersampling, known as point-scanning super-resolution (PSSR) imaging, can mitigate these limitations. PSSR facilitates high-resolution, fast, and sensitive image acquisition with otherwise unattainable resolution.
Point-scanning imaging systems are among the most widely used tools for high-resolution cellular and tissue imaging, benefiting from arbitrarily defined pixel sizes. The resolution, speed, sample preservation and signal-to-noise ratio (SNR) of point-scanning systems are difficult to optimize simultaneously. We show these limitations can be mitigated via the use of deep learning-based supersampling of undersampled images acquired on a point-scanning system, which we term point-scanning super-resolution (PSSR) imaging. We designed a 'crappifier' that computationally degrades high SNR, high-pixel resolution ground truth images to simulate low SNR, low-resolution counterparts for training PSSR models that can restore real-world undersampled images. For high spatiotemporal resolution fluorescence time-lapse data, we developed a 'multi-frame' PSSR approach that uses information in adjacent frames to improve model predictions. PSSR facilitates point-scanning image acquisition with otherwise unattainable resolution, speed and sensitivity. All the training data, models and code for PSSR are publicly available at 3DEM.org.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available