4.8 Article

Imaging-based screens of pool-synthesized cell libraries

Journal

NATURE METHODS
Volume 18, Issue 4, Pages 358-365

Publisher

NATURE PORTFOLIO
DOI: 10.1038/s41592-020-01053-8

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Funding

  1. Knut and Alice Wallenberg Foundation (KAW)

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Linking genetic perturbations to changes in phenotype is crucial in biological research. Advances in microscopy and the creation of genetically altered cell libraries have allowed for live-cell imaging of pool-generated strain libraries, enhancing biological discovery.
Mapping a genetic perturbation to a change in phenotype is at the core of biological research. Advances in microscopy have transformed these studies, but they have largely been confined to examining a few strains or cell lines at a time. In parallel, there has been a revolution in creating synthetic libraries of genetically altered cells with relative ease. Here we describe methods that combine these powerful tools to perform live-cell imaging of pool-generated strain libraries for improved biological discovery.

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