Journal
NATURAL PRODUCT COMMUNICATIONS
Volume 16, Issue 2, Pages -Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/1934578X21993335
Keywords
capsaicin; 5-fluorouracil; thymidylate synthase; p38 MAPK; non-small cell lung cancer
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Funding
- Ministry of Science and Technology, Taiwan [MOST 108-2320-B-415-004 -, MOST 109-2314-B-002-175 -]
- Ditmanson Medical Foundation Chia-Yi Christian Hospital Research Program [R109-38]
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The study showed that capsaicin in combination with 5-FU exhibits synergistic anticancer effects on NSCLC cells by downregulating TS expression and inhibiting cell growth through p38 MAPK inactivation.
Capsaicin, an ingredient of green and red bell peppers, shows anticancer activity in several malignant cell lines. Thymidylate synthase (TS) is a well-validated anticancer drug target in non-small cell lung cancer (NSCLC) cells. However, whether capsaicin and 5-fluorouracil (5-FU) induce synergistic cytotoxicity in NSCLC cells by regulating TS expression is unclear. This study investigated the cytotoxicity of capsaicin and 5-FU co-treatment on two hoursuman lung adenocarcinoma cell lines, H520 and H1703, and the underlying mechanisms. Capsaicin decreased TS expression in a p38 mitogen-activated protein kinase (MAPK) inactivation-dependent manner in H520 and H1703 cells. Enhancement of p38 MAPK activity by transfection with constitutive active mitogen-activated protein kinase kinase six vectors increased TS expression and cell survival. In addition, capsaicin and 5-FU co-treatment enhanced synergistic cytotoxicity and inhibited cell growth associated with TS downregulation and p38 MAPK inactivation in H520 and H1703 cells. Capsaicin and 5-FU co-treatment did not affect the cellular content of capsaicin. These results show that capsaicin may be combined with 5-FU to treat NSCLC.
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