Imaging of Cell Morphology Changes via Metamaterial-Assisted Photobleaching Microscopy

Determining the axial position of an emitter with nanoscale precision is critical to a fundamental imaging methodology. While there are many advanced optical techniques being applied to high-resolution imaging, high-axial-resolution topography imaging of living cells is particularly challenging. Here, we present an application of metamaterial-assisted photobleaching microscopy (MAPM) with high-axial resolution to characterize morphological properties of living cells. Quantitative imaging of changes in the morphology of live cells is obtained by topographic and statistical analysis. The time-lapse topography image using the metamaterial-induced photostability provides information about growth factor induced changes in the cell morphology with high-axial resolution.

Automated summary

This study presents an application of metamaterial-assisted photobleaching microscopy (MAPM) with high-axial resolution to characterize morphological properties of living cells, providing quantitative imaging of changes in cell morphology through topographic and statistical analysis. The time-lapse topography image obtained using metamaterial-induced photostability offers insights into growth factor induced changes in cell morphology with high-axial resolution.