Journal
NANO LETTERS
Volume 21, Issue 4, Pages 1888-1895Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.1c00094
Keywords
extracellular vesicles; exosomes; LNP; RNA therapeutics; nanomedicine
Categories
Funding
- European Union's Horizon 2020 Research and Innovation program in the project B-SMART [721058]
- VENI Fellowship from the Dutch Research Council (NWO) [VI.Veni.192.174]
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RNA therapeutics have great potential, but face challenges in delivery to target cells. Extracellular vesicles (EVs) have emerged as promising RNA delivery vehicles with significantly higher delivery efficiency compared to synthetic systems.
RNA therapeutics have high potential that is yet to be fully realized, largely due to challenges involved in the appropriate delivery to target cells. Extracellular vesicles (EVs) are lipid bound nanoparticles released by cells of all types and possess numerous features that may help overcome this hurdle and have emerged as a promising RNA delivery vehicle candidate. Despite extensive research into the engineering of EVs for RNA delivery, it remains unclear how the intrinsic RNA delivery efficiency of EVs compares to currently used synthetic RNA delivery vehicles. Using a novel CRISPR/Cas9-based RNA transfer reporter system, we compared the delivery efficiency of EVs to clinically approved state-of-the-art DLin-MC3-DMA lipid nanoparticles and several in vitro transfection reagents. We found that EVs delivered RNA several orders of magnitude more efficiently than these synthetic systems. This finding supports the continued research into EVs as potential RNA delivery vehicles.
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